Complete covalent structure of human .beta.-thromboglobulin

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Abstract
The complete primary structure of the platelet-specific protein human .beta.-thromboglobulin was determined. .beta.-Thromboglobulin consists of identical subunits of 81 amino acids, each with a MW of 8851. The amino acid sequence of the .beta.-thromboglobulin subunit is as follows: Gly-Lys-Glu-Glu-Ser-Leu-Asp-Ser-Asp-Leu-Tyr-Ala-Glu-Leu-Arg-Cys-Met-Cys-Ile-Lys-Thr-Thr-Ser-Gly-Ile-His-Pro-Lys-Asn-Ile-Gln-Ser-Leu-Glu-Val-Ile-Gly-Lys-Gly-Thr-His-Cys-Asn-Gln-Val-Glu-Val-Ile-Ala-Thr-Leu-Lys-Asp-Gly-Arg-Lys-Ile-Cys-Leu-Asp-Pro-Asp-Ala-Pro-Arg-Ile-Lys-Lys-Ile-Val-Gln-Lys-Lys-Leu-Ala-Gly-Asp-Glu-Ser-Ala-Asp. Disulfide bridges link half-cystine-16 to half-cystine-42, and half-cystine-18 to half-cystine-58. The amino acid sequence of .beta.-thromboglobulin shows a marked homology with that of platelet factor 4. When the sequences are aligned for maximum homology, 42 of the 81 residues of .beta.-thromboglobulin are identical with those of platelet factor 4, including the position of the 4 half-cystines.