Purification and characterization of a cell wall proteinase fromStreptococcus lactisNCDO 763
- 1 May 1987
- journal article
- research article
- Published by Cambridge University Press (CUP) in Journal of Dairy Research
- Vol. 54 (2), 247-255
- https://doi.org/10.1017/s0022029900025383
Abstract
A proteinase was purified from a cell wall extract of a culture of Streptococcus lactis NCDO 763 grown in skim milk. Being active at a low pH (at pH 4·8 on haemoglobin and pH 6·–6·5 on casein) and completely inhibited by diisopropylfluorophosphate, it was considered to be a serine proteinase partly inhibited by EDTA; the mol. wt was ∼ 80000.This publication has 18 references indexed in Scilit:
- Partial characterization and comparison of cell wall proteinases from 5 strains of Streptococcus lactisLe Lait, 1987
- Simultaneous extraction and purification of a cell wall-associated peptidase and β-casein specific proteas fromStreptococcus cremoris AC1Applied Microbiology and Biotechnology, 1985
- Discontinuous polyacrylamide gel electrophoresis of cell wall proteinase from variants ofStreptococcus lactisJournal of Applied Bacteriology, 1985
- A Dual-Directed Control of Cell Wall Proteinase Production in Streptococcus cremoris AM1: A Possible Mechanism of Regulation During Growth in MilkJournal of Dairy Science, 1985
- [30] Gel protein stains: Silver stainMethods in Enzymology, 1984
- 14C-methylated β-casein as a substrate for plasmin, and its application to the study of milk protein transformationsBiochimica et Biophysica Acta (BBA) - Protein Structure, 1980
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Tagatose-1,6-diphosphate activation of lactate dehydrogenase from Streptococcus cremorisBiochemical and Biophysical Research Communications, 1975
- Inventaire des différentes activités peptidasiques intracellulaires de Streptococcus thermophilus: Purification et propriétés d'une dipeptide-hydrolase et d'une aminopeptidaseBiochimie, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970