Nitric Oxide Modulates the Expression of Monocyte Chemoattractant Protein 1 in Cultured Human Endothelial Cells
- 1 June 1995
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation Research
- Vol. 76 (6), 980-986
- https://doi.org/10.1161/01.res.76.6.980
Abstract
The recruitment of monocytes into the arterial wall is one of the earliest events in the pathogenesis of atherosclerosis. Since monocyte chemoattractant protein 1 (MCP-1) plays a key role in the subendothelial recruitment of monocytes, we tested whether nitric oxide (NO) modulates the expression of MCP-1 in cultured human endothelial cells. Inhibition of basal NO production by NG-nitro-l-arginine (L-NAG) upregulates endothelial MCP-1 mRNA expression (250±20%) and protein secretion. Exogenous addition of NO dose-dependently decreased MCP-1 mRNA expression and secretion. Changes in MCP-1 mRNA expression and protein secretion were paralleled by corresponding changes in chemotactic activity of cell-conditioned media for monocytes. An MCP-1 antibody reduced monocyte chemotactic activity by 85% and completely abolished the increased monocyte chemotactic activity induced by the inhibition of NO production. Elevation of endothelial cGMP levels had no significant effect on MCP-1 mRNA expression. Inhibitio...Keywords
This publication has 45 references indexed in Scilit:
- Activation of MCP-1 Gene Expression Is Mediated through Multiple Signaling PathwaysBiochemical and Biophysical Research Communications, 1993
- Biosynthesis and Metabolism of Endothelium-Derived Nitric OxideAnnual Review of Pharmacology and Toxicology, 1990
- Release of endothelin from the porcine aorta. Inhibition by endothelium-derived nitric oxide.JCI Insight, 1990
- Evidence for the presence of oxidatively modified low density lipoprotein in atherosclerotic lesions of rabbit and man.JCI Insight, 1989
- Monocyte chemotactic protein gene expression by cytokine-treated human fibroblasts and endothelial cellsBiochemical and Biophysical Research Communications, 1989
- Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells.JCI Insight, 1989
- Human monocyte chemoattractant protein‐1 (MCP‐1) Full‐length cDNA cloning, expression in mitogen‐stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JEFEBS Letters, 1989
- Multiple nuclear factors interact with the immunoglobulin enhancer sequencesCell, 1986
- The Pathogenesis of Atherosclerosis — An UpdateNew England Journal of Medicine, 1986
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970