High-Speed DNA Genotyping Using Microfabricated Capillary Array Electrophoresis Chips
- 1 June 1997
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 69 (11), 2181-2186
- https://doi.org/10.1021/ac961237+
Abstract
Capillary array electrophoresis (CAE) chips have been designed and fabricated with the capacity to rapidly (MspI DNA samples. Genotyping of HLA-H, a candidate gene for the diagnosis of hereditary hemochromatosis, was then performed to demonstrate the rapid analysis of biologically relevant samples. Two-color multiplex fluorescence detection of HLA-H genotypes was accomplished by prelabeling the standard pBR322 MspI DNA ladder with a red emitting bis-intercalation dye (butyl TOTIN) and on-column labeling of the HLA-H DNA with thiazole orange. This work establishes the feasibility of using CAE chips for high-speed, high-throughput genotyping.Keywords
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