Rat preprocarboxypeptidase A: cDNA sequence and preliminary characterization of the gene.
- 1 January 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (1), 31-35
- https://doi.org/10.1073/pnas.79.1.31
Abstract
Rat carboxypeptidase A c[complementary]DNA clones were isolated from a cDNA library prepared from pancreatic mRNA. An almost complete mRNA sequence was deduced predicting a polypeptide with 78% amino acid sequence homology with bovine carboxypeptidase A. The amino acid sequence of the activation and signal peptides of the carboxypeptidase A precursor were inferred from the nucleotide sequence. The cDNA was used as a probe to identify DNA fragments containing carboxypeptidase A sequences in a bacteriophage .lambda. library of rat genomic DNA. Heteroduplexes revealed that the DNA coding sequence occupies 5.5 kilobases and is interrupted by 9 intervening sequences. The nucleotide sequence of the 5'' end of the gene and the adjacent flanking region provides information on the site of initiation of transcription and the putative control regions. There is no evident relationship between the localization of intervening sequences in the gene and functional/structural domains of the protein.This publication has 22 references indexed in Scilit:
- Why genes in pieces?Nature, 1978
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Novel Screening Procedure for Recombinant PlasmidsScience, 1977
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Homology in amino-terminal sequence of precursors to pancreatic secretory proteins.Proceedings of the National Academy of Sciences, 1975
- Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.The Journal of cell biology, 1975
- Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.Proceedings of the National Academy of Sciences, 1975
- Nucleotide sequence of the rightward operator of phage lambda.Proceedings of the National Academy of Sciences, 1975
- The structure of carboxypeptidase A. VIII. Atomic interpretation at 0.2 nm resolution, a new study of the complex of glycyl-L-tyrosine with CPA, and mechanistic deductionsPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1970
- THE AMINO ACID SEQUENCE OF BOVINE CARBOXYPEPTIDASE AProceedings of the National Academy of Sciences, 1969