Chloroquine Removal of HLA Antigens from Platelets for the Platelet Immunofluorescence Test
- 5 March 1985
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 48 (3), 156-159
- https://doi.org/10.1111/j.1423-0410.1985.tb00163.x
Abstract
Chloroquine-treated [human] platelets did not react with HLA antisera in a platelet suspension immunofluorescence test (PSIFT), while the reactions with platelet-specific antibodies (P1A1, Baka) seemed even stronger after treatment. The method might be useful in discerning HLA and platelet-specific antibodies without absorption procedures. The treatment causes death of a proportion of the cells, with nonspecific fluorescence making the reading difficult. This may be circumvented by using special phase-contrast lenses.This publication has 9 references indexed in Scilit:
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