Studies on histones. 4. The histones of wheat germ

Abstract

A process for the extrac-tion of histones from wheat germ is given. A partial fractionation with acetone is described; the fractions were examined by amino acid analysis and by starch-gel electrophoresis. The fraction corresponding to the arginine-rich histones of animal tissues appears to be missing, but a lysine-rich and a moderately lysine-rich histone have been obtained. These components differ markedly from similar fractions from animal tissue; e.g. the predominant N-terminal group in both cases is alanine, which is the N-terminal of the arginine-rich histones of the animal tissues examined.