Fidelity of replication of phage phi X174 DNA by DNA polymerase III holoenzyme: spontaneous mutation by misincorporation.
- 1 October 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (10), 4946-4950
- https://doi.org/10.1073/pnas.76.10.4946
Abstract
DNA from .vphi.X174 is replicated in vitro with a fidelity similar to that found genetically. A mutation of TAG .fwdarw. TGG may be induced by varying the concentrations of deoxynucleoside triphosphates, with a frequency proportional to [dGTP]2/[dATP]. This complex concentration dependence is consistent with the active participation of a proofreading mechanism that hydrolytically excises mismatched base pairs as they are formed. A simple kinetic analysis predicts that the frequency of misincorporation depends on the ratio of incorrect to correct deoxynucleoside triphosphates times the concentration of the next triphosphate in the sequence to be added. Spontaneous mutation by misincorporation may depend crucially on the composition of the deoxynucleoside triphosphate pool.This publication has 24 references indexed in Scilit:
- Probing DNA Replication Mechanisms with the T4 Bacteriophage In Vitro SystemPublished by Cold Spring Harbor Laboratory ,1979
- Purification of the rep protein of Escherichia coli. An ATPase which separates duplex DNA strands in advance of replication.Journal of Biological Chemistry, 1978
- Transfection of Enterobacteriaceae and its applications.1978
- A mechanism of duplex DNA replication revealed by enzymatic studies of phage phi X174: catalytic strand separation in advance of replication.Proceedings of the National Academy of Sciences, 1977
- Enzymatic replication of viral and complementary strands of duplex DNA of phage phiX174 proceeds by seprate mechanisms.Proceedings of the National Academy of Sciences, 1976
- The mechanism of replication of øX174 DNA: XII. Non-random location of gaps in nascent øX174 RF II DNAJournal of Molecular Biology, 1975
- Partition analysis and concept of net rate constants as tools in enzyme kineticsBiochemistry, 1975
- The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication.Journal of Biological Chemistry, 1975
- Ion-exchange chromatography of nucleotides on poly-(ethyleneimine)-cellulose thin layersJournal of Chromatography A, 1964
- Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174.1963