Quantitative Study on Anomeric Forms of Glucose Produced by α-Glucosidases1

Abstract

Anomeric forms of glucose produced from phenyl α-maltoside, maltose, or phenyl a-glucoside have been determined quantitatively by simultaneous measurements of optical rotation and reducing power, for eight kinds of glucose-producing 1,4-α-glucosyl hydrolases, including glucose-forming amylase from human urine, and α-glucosidases from pig serum, honey bee, buckwheat seed, rice seed, sugar beet seed, flint corn seed, and brewer's yeast. All the eight enzymes studied were found to produce α-glucose exclusively.