Abstract
An accurate method for the assay of bacterial hyaluronidase from the liberation of reducing sugars from K hyaluronate was described. The pH optima and relative potencies of streptococcal and staphylococcal hyaluronidase by this method did not agree with measurements made by the viscosity-reducing technic and it was deduced that prepns. of streptococcal and staphylococcal hyaluronidase contained several enzymes responsible for various stages in the degradation. Highly potent hyaluronidase was produced by growing streptococci inside dialysis sacs.