Assay of Anticoagulant Unit of ω-Heparin Newly Isolate from Whale's Lung and Intestine and its Toxicity

Abstract

Authors developed a modified assay method for standardization of anticoagulant activity of whale heparin by use of the mixture of human and ox sulphated blood and ox thromboklnase. The stable unit was obtained by this method in the course of preservation of mixed sulphated blood at 4[degree]C for 20 days. The toxicity of whale heparin was the same as that of beef heparin. Tests of the contamination of histamine and pyrogenic substance were negative.