DNA-PROTEIN AND DNA INTERSTRAND CROSS-LINKING BY CIS-PLATINUM(II) AND TRANS-PLATINUM(II) DIAMMINEDICHLORIDE IN L1210 MOUSE LEUKEMIA-CELLS AND RELATION TO CYTOTOXICITY
- 1 January 1979
- journal article
- research article
- Vol. 39 (2), 365-369
Abstract
The effects of cis- and trans-platinum(II) diamminedichloride on L1210 [mouse leukemia] cells were investigated using the technique of DNA alkaline elution. Both produced reductions in alkaline elution rates which were partially or, in some cases, almost completely reversed by incubation of the cell lysates with proteinase K. The proteinase-sensitive component of this effect reflects DNA-protein cross-linking, while the proteinase-resistant component of this effect may include interstrand cross-links. The cytotoxicity of the 2 agents did not correlate with the extent of DNA-protein cross-linking but did appear to correlate with the extent of proteinase-resistant cross-linking. There may be a relationship between cytotoxicity and interstrand cross-linking.This publication has 17 references indexed in Scilit:
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