A New Surface Antigen on Human Lymphocytes Defined by the Murine Monoclonal Antibody IVF7
- 1 April 1990
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 31 (4), 461-467
- https://doi.org/10.1111/j.1365-3083.1990.tb02793.x
Abstract
The murine monoclonal antibody (MoAb) IVF7 was produced against tumour cells from a patient with a CD3+, CD4+, CD8- T-cell chronic lymphatic leukaemia (T-CLL). The MoAb IVF7 showed reactivity with subpopulations of normal peripheral blood lymphocytes (PBL), as well as with a few cell lines of haematopoietic origin. Thirty-six per cent of PBL were stained with IVF7. Analysing subpopulations, we found that 80% of NK cells, 25% of T cells, and 10-20% of B cells were positive. The myelomonocytic cell line KG-1 was also stained. The molecular weight of the molecule was 40 kDa under reducing conditions. The antigen was found to be trypsin-sensitive. MoAb IVF7 could modulate the antigen from the cell surface. The antibody did not stimulate PBL to DNA synthesis, nor did it significantly influence NK cell-mediated killing.This publication has 10 references indexed in Scilit:
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