Localization of the Enzymatic Block in Kidneys of Rats Treated with Fluoroacetate

Abstract

Oxidation of citrate was markedly depressed in homogenates of kidneys from rats treated with fluoroacetate, but oxidation of other substrates of the Krebs cycle, including cis-aconitate, was depressed only to a relatively small extent. No inhibition of aconitase was found when cis-aconitate was the substrate. Evidently its conversion to citrate was relatively unaffected under conditions which strongly inhibited the reverse reaction.