Elongation of displacement-loop strands in human and mouse mitochondrial DNA is arrested near specific template sequences.
- 1 October 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (10), 6116-6120
- https://doi.org/10.1073/pnas.78.10.6116
Abstract
Animal mitochondrial [mt] DNA maintains a displacement loop (D loop) at the heavy strand origin of replication. These D loops represent sharply limited synthesis of heavy strands and provide a unique opportunity to examine the termination of DNA synthesis. Direct sizing at the nucleotide level indicates that the 3'' ends of D-loop strands of human and mouse mtDNA are discrete and map within 3-5 nucleotides on the complementary template strand. In the case of human mtDNA, there is a single trinucleotide stop point 51-53 nucleotides downstream from a 15-nucleotide template sequence (3''T-A-A-C-C-C-A-A-A-A-A-T-A-C-A 5'') which is repeated 4 times in the mouse mtDNA D loop region 3''(T-A-A-Py-Py-A-A-A-T-T-A-C-A 5''). The stop points of the 5 major mouse D-loop strands are 24-63 nucleotides downstream from the 4 repeated template sequences. The arrest of D-loop strand elongation is an event determined by template sequence.This publication has 15 references indexed in Scilit:
- Sequence and organization of the human mitochondrial genomeNature, 1981
- Precise localization and nucleotide sequence of the two mouse mitochondrial rRNA genes and three immediately adjacent novel tRNA genesCell, 1980
- 3′-End labeling of DNA with [α-32P] cordycepin-5′-triphosphateGene, 1980
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesMethods in Enzymology, 1980
- Mechanism of mitochondrial DNA replication in mouse L-cells: RNA priming during the initiation of heavy-strand synthesisJournal of Molecular Biology, 1979
- Mechanism of mitochondrial DNA replication in mouse L-cells: Kinetics of synthesis and turnover of the initiation sequenceJournal of Molecular Biology, 1978
- Human mitochondrial DNA: analysis of 7S DNA from the origin of replication.Proceedings of the National Academy of Sciences, 1978
- Displacement-loop replication initiation sequence in animal mitochondrial DNA exists as a family of discrete lengthsProceedings of the National Academy of Sciences, 1978
- Mechanism of mitochondrial DNA replication in mouse L-cells: Asynchronous replication of strands, segregation of circular daughter molecules, aspects of topology and turnover of an initiation sequenceJournal of Molecular Biology, 1974
- A Novel Closed-Circular Mitochondrial DNA with Properties of a Replicating IntermediateProceedings of the National Academy of Sciences, 1971