Primary structures of new ‘iso‐hirudins’
- 11 September 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 255 (1), 105-110
- https://doi.org/10.1016/0014-5793(89)81070-1
Abstract
Crude hirudin (12.7 U/micrograms), a complex mixture of polypeptides obtained from the leech, could be separated by microbore HPLC. A combination of amino acid analysis, N-terminal microsequencing and chemical as well as enzymatic fragmentation made the primary sequence of the new isohirudins Ia-IIIb' accessible. The biological activity determined in the thrombin inhibition test showed a comparable value for all of these compounds. The results presented address the question as to whether these isohirudins are true mutations from a family of genes or a family of leeches.Keywords
This publication has 13 references indexed in Scilit:
- Anion-binding exosite of human .alpha.-thrombin and fibrin(ogen) recognitionBiochemistry, 1988
- Identification of regions of .alpha.-thrombin involved in its interaction with hirudinBiochemistry, 1987
- Cloning and expression of a cDNA coding for the anticoagulant hirudin from the bloodsucking leech, Hirudo medicinalis.Proceedings of the National Academy of Sciences, 1986
- On the isolation of the thrombin inhibitor hirudinThrombosis Research, 1985
- The Complete Covalent Structure of Hirudin. Localization of the Disulfide BondsBiological Chemistry Hoppe-Seyler, 1985
- The complete amino acid sequence of hirudin, a thrombin specific inhibitorFEBS Letters, 1984
- Hirudin from leech heads and whole leeches and “pseudo-hirudin” from leech bodiesThrombosis Research, 1983
- [14] Cleavage at AsnGly bonds with hydroxylamineMethods in Enzymology, 1977
- Reindarstellung und Analyse des Thrombininhibitors HirudinHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1967
- On the Determination of Cystine as Cysteic AcidJournal of Biological Chemistry, 1963