Crystal structure of a yeast TBP/TATA-box complex
- 7 October 1993
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 365 (6446), 512-520
- https://doi.org/10.1038/365512a0
Abstract
The 2.5 Å crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA.Keywords
This publication has 43 references indexed in Scilit:
- Crystal structure of TFIID TATA-box binding proteinNature, 1992
- Transcription factor TFIID induces DNA bending upon binding to the TATA element.Proceedings of the National Academy of Sciences, 1992
- Interaction of TFIID in the minor groove of the TATA elementCell, 1991
- TFIID binds in the minor groove of the TATA boxCell, 1991
- A highly conserved domain of TFIID displays species specificity in vivoCell, 1991
- Dominant negative mutations in yeast TFIID define a bipartite DNA-binding regionCell, 1991
- Functional differences between yeast and human TFIID are localized to the highly conserved regionCell, 1991
- Isolation and characterization of the Drosophila gene encoding the TATA box binding protein, TFIIDCell, 1990
- Five intermediate complexes in transcription initiation by RNA polymerase IICell, 1989
- Physical Analysis of Transcription Preinitiation Complex Assembly on a Class II Gene PromoterScience, 1988