Distinct epitopes on Ik gene products identified by monoclonal antibodies
- 1 December 1980
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 10 (12), 950-957
- https://doi.org/10.1002/eji.1830101211
Abstract
Analysis of reactivity of monoclonal anti-Iak alloantibodies, obtained by fusion between NS 1 myeloma and spleen cells from alloimmune A.TH mice, permitted the identification of 9 distinct determinants of the Ik gene products. Competitive binding experiments indicated that 2 private epitopes (defined by H 8–109.13 and H 8–138.4 antibodies) of the I-Ak product could be separated, thereby apparently splitting the Ia.2 specificity. A public determinant of the I-Ak molecule (identified by H 8–15.9 antibody) was found expressed not only on the 1-A products of the H-2b, H-2d, H-2ja, H-2P and H-2q murine haplotypes, but also on human HLA-DR antigens. Four determinants of the I-E/Ck antigen (defined by H 7–8.26, H 10–81.10, H 10–93.2 and H 8–86.2 antibodies) had a strain distribution analogous to the Ia.7 specificity. However, competitive binding experiments, and the cross-reactivity pattern with la-like antigens from other species (e.g. human HLA-DR antigens) indicated that these antibodies detected distinct determinants on the I-E/Ck molecule, thereby subdividing the broad Ia.7 specificity. Two other determinants (defined by H9–14.8 and H9–15.4 antibodies) had a strain distribution that did not permit a precise assignment to a given Ia antigen, even though preliminary data suggested that they could detect separate determinants on the I-E/Ck product. All these monoclonal antibodies identified membrane antigens with the expected Ia tissue distribution pattern, and most of them could precipitate a molecule containing two chains of 28 kD and 35 kD, from mouse spleen cell lysates.This publication has 30 references indexed in Scilit:
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