Effects of anti‐triadin antibody on Ca2+ release from sarcoplasmic reticulum

Abstract

The monoclonal antibody, mAb GE 4.90, raised against triadin, a 95 kDa protein of sarcoplasmic reticulum (SR), inhibits the slow phase of Ca²⁺ release from SR following depolarization of the T-tubule moiety of the triad. The antibody has virtually no effect on the fast phase of depolarization-induced Ca²⁺ release nor on caffeine-induced Ca²⁺ release. Since the slow phase of depolarization-induced Ca²⁺ release is also inhibited by dihydropyridines (DHP), these results suggest that triadin may be involved in the functional coupling between the DHP receptor and the SR Ca²⁺ channel.