THE GENE FOR CYSTATHIONINE BETA-SYNTHASE (CBS) MAPS TO THE SUBTELOMERIC REGION ON HUMAN CHROMOSOME-21Q AND TO PROXIMAL MOUSE CHROMOSOME-17

  • 1 April 1988
    • journal article
    • research article
    • Vol. 42 (4), 550-559
Abstract
The human gene for cystathionine .beta.-synthase (CBS), the enzyme deficient in classical homocystinuria, has been assigned to the subtelomeric region of band 21q22.3 by in situ hybridization of a rat cDNA probe to structurally rearranged chromosomes 21. The homologous locus in the mouse (Cbs) was mapped to the proximal half of mouse chromosome 17 by Southern analysis of Chinese hamster .times. mouse somatic cell hybrid DNA. Thus, CBS/Cbs and the gene for .alpha.A-crystalline (CRYA1/Crya-1 or Acry-1) form a conserved linkage group on human (HSA) chromosome region 21q22.3 and mouse (MMU) chromosome 17 region A-C. Features of Down syndrome (DS) caused by three copies of these genes should not be present in mice trisomic for MMU 16 that have been proposed as animal models for DS. Mice partially trisomic for MMU 16 or MMU 17 should allow gene-specific dissection of the trisomy 21 phenotype.