Covalent linkage of the DNA repair template to the CRISPR/Cas9 complex enhances homology-directed repair
Preprint
- 11 November 2017
- preprint
- Published by Cold Spring Harbor Laboratory in bioRxiv
- p. 218149
- https://doi.org/10.1101/218149
Abstract
The CRISPR/Cas9 targeted nuclease technology allows the insertion of genetic modifications with single base-pair precision. The preference of mammalian cells to repair Cas9-induced DNA double-strand breaks via non-homologous end joining (NHEJ) rather than via homology-directed repair (HDR) however leads to relatively low rates of correctly edited loci. Here we demonstrate that covalently linking the DNA repair template to Cas9 increases the ratio of HDR over NHEJ up to 23-fold, and therefore provides advantages for clinical applications where high-fidelity repair is needed.All Related Versions
- Published version: eLife, 7.
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