Chemical reactions which affect the biological activity of human gonadotrophins
- 1 April 1961
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 79 (1), 64-70
- https://doi.org/10.1042/bj0790064
Abstract
The effects of various reagents on the activity of human urinary and pituitary gonadotrophins has been studied by 2 assay methods, the first measuring the combined effects of follicle-stimulating hormone and interstitial-cell-stimulating hormone and the second being more specific for follicle-stimulating hormone. The only reagent to affect one type of activity more than the other was sodium borohydride. This appeared to destroy interstitial-cell-stimulating hormone more readily than follicle-stimulating hormone. Methods are described for the combination of urinary gonadotrophin with Zn, Cu and triazine dyes without loss of biological activity.Keywords
This publication has 16 references indexed in Scilit:
- THE USE OF ION-EXCHANGE MATERIALS IN THE FRACTIONATION OF GONADOTROPHINS FROM URINEActa Endocrinologica, 1959
- Purification of Human Pituitary Follicle Stimulating (FSH) and Luteinizing (LH) Hormones.Experimental Biology and Medicine, 1959
- Labelling of Proteins with Cellulose-reactive DyesNature, 1959
- Differences between Duplicate Assays of Human Urinary GonadotrophinsNature, 1958
- Nouvelle méthode de purification de l'hormone gonadotrope sérique de jument gravideBiochimica et Biophysica Acta, 1958
- THE REACTION OF SEVERAL PROTEIN HORMONES WITH PERIODATE1Endocrinology, 1958
- THE EXTRACTION OF GONADOTROPHINS FROM URINE BY THE COMBINED USE OF BENZOIC ACID AND TUNGSTIC ACIDJournal of Endocrinology, 1958
- THE USE OF ION-EXCHANGE MATERIALS IN THE PREPARATION OF GONADOTROPHINS FROM URINEJournal of Endocrinology, 1958
- Preparation And Concentration Of The Pituitary Gonadotropins From Human PlasmaJournal of Biological Chemistry, 1957
- A chemical method for the determination of oestriol, oestrone and oestradiol in human urineBiochemical Journal, 1955