Diffusion Limitation of Oxygen Uptake and Nitrogenase Activity in the Root Nodules of Parasponia rigida Merr. and Perry

Abstract

Export of labeled material derived by continuous photosynthesis in ¹⁴CO₂ was monitored with a Geiger-Müller detector positioned next to an exporting leaf blade. Rate of export of labeled material was calculated from the difference between rates of retention and net photosynthesis of labeled carbon for the observed leaf. Given certain conditions, including nearly constant distribution of labeled material among minor veins and various types of cells, count rate data for the source leaf can be converted to rate of export of carbon. Changes in counting efficiency resulting from changes in leaf water status can be corrected for with data from a transducer which measures leaf thickness.