Serological and immunochemical analysis of the products of a single HLA DR-alpha and DR-beta chain gene expressed in a mouse cell line after DNA-mediated cotransformation reveals that the beta chain carries a known supertypic specificity.
Open Access
- 1 July 1985
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 162 (1), 105-116
- https://doi.org/10.1084/jem.162.1.105
Abstract
Using a mouse cell line transformed with and expressing a single HLA DR-.alpha. and DR-.beta. chain gene, evidence is presented that the product of the DR-.beta. chain gene carries a supertypic determinant, BR3, previously defined by serology. The amino acid sequence of this .beta. chain gene is determined from the DNA sequence. Another DR-associated supertypic specificity defined by monoclonal antibody MCS7 was not encoded by this DR-.beta. chain gene. This provides formal proof that a supertypic specificity can be associated with a product of a distinct DR-.beta. locus. Haplotypes sharing such specificites may be evolutionarily related.This publication has 27 references indexed in Scilit:
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