A murine cell line (2E10.4.13) produces five hemopoietic stimulators, and interleukin-2 and interleukin-3.
- 1 January 1985
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 10 (2), 121-131
- https://doi.org/10.1247/csf.10.121
Abstract
An interleukin-2 (IL-2)-independent murine lymphocyte clone (2E10.4.13) with the Thyl+Lyt1+2-T200+ phenotype was separated from theoriginal IL-2-dependent natural killer (NK) cell line (PEC-1). Erythroid burst-promoting activity (BPA), erythropoietin (Ep), granulocyte/macrophage, megakaryocyte and eosinophil colony-stimulating factors (GM-, MK-and Eo-CSF), IL-2 and Interleukin-3 (IL-3) were produced when these cells were stimulated with phorbol myristate acetate (PMA). When the conditioned medium was run through ion-exchange high-performance liquid chromatography, BPA, Ep, GM-CSF, MK-CSF and Eo-CSF were eluted in the same region as IL-3. In contrast, MK-CSF, much of the GM-CSF and half of the Eo-CSF were eluted in a distinct region where no IL-3 was detected. Chemical analyses of the hemopoietic factors derived from a single T inducer clone indicated that all the hemopoietic activities were associated with IL-3 activity. Some CSF activities (GM-, MK-and Eo-CSF) also could be mediated by the distinct molecules from IL-3, evidence that heterogeneous molecules are responsible for CSF activity.This publication has 4 references indexed in Scilit:
- Biologic properties of homogeneous interleukin 3. I. Demonstration of WEHI-3 growth factor activity, mast cell growth factor activity, p cell-stimulating factor activity, colony-stimulating factor activity, and histamine-producing cell-stimulating factor activity.The Journal of Immunology, 1983
- MORPHOLOGICAL EXAMINATIONS OF MURINE GRANULOCYTE MACROPHAGE COLONIES GROWN IN SERUM-FREE CULTURES1983
- Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterolExperimental Cell Research, 1980
- NEW TECHNIQUE FOR THE CYTOCHEMICAL EXAMINATION OF HUMAN HEMATOPOIETIC-CELLS GROWN IN AGAR-GEL1980