The Ion Interaction Reversed Phase High-Performance Liquid Chromatography and the Voltammetry With Microelectrodes In the Analysis of Milk

Abstract

Some anionic species present at the natural pH of milk (6.72), namely hydrogen-orthophosphate, L(+)-ascorbate and hydrogencitrate were detected in samples of crude bovine-milk both by ion interaction reverse phase HPLC chromatography and by amperometry based on microelectrodes. The RSD ranged between 2 and 5 % for the former technique and between 3 and 4 % for the latter. the accuracy was evaluated by comparing the data obtained with the two techniques and discrepancies between the methods never exceeded 9% .