Phosphonoacetic Acid-Resistant Mutants of Herpes Simplex Virus: Effect of Phosphonoacetic Acid on Virus Replication and In Vitro Deoxyribonucleic Acid Synthesis in Isolated Nuclei
- 1 May 1977
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 11 (5), 919-922
- https://doi.org/10.1128/aac.11.5.919
Abstract
Phosphonoacetic acid (PAA) inhibits the replication of herpes simplex virus in BSC-1 cells and the in vitro synthesis of deoxyribonucleic acid (DNA) in isolated nuclei. Phosphonopropionic acid at a concentration of 100 μg/ml had no effect on herpes simplex virus replication. PAA-resistant mutants were obtained at a rate of 1 in 104 plaque-forming units after 5-bromodeoxyuridine mutagenization of the virus. These mutants replicate in BSC-1 cells in the presence of 100 μg of PAA per ml and induce a PAA-resistant DNA polymerase that synthesizes DNA in vitro in the presence of PAA.Keywords
This publication has 11 references indexed in Scilit:
- Mutants of Herpes Simplex Virus Types 1 and 2 that are Resistant to Phosphonoacetic Acid Induce Altered DNA Polymerase Activities in Infected CellsJournal of General Virology, 1976
- Phosphonoacetic Acid in the Treatment of Experimental Ocular Herpes Simplex InfectionsAntimicrobial Agents and Chemotherapy, 1976
- Mechanism of phosphonoacetate inhibition of herpesvirus-induced DNA polymeraseBiochemistry, 1976
- Mode of inhibition of Herpes simplex virus DNA polymerase by phosphonoacetateBiochemistry, 1975
- Analysis of herpes simplex virus DNA synthesized in vitro in nuclei isolated from infected BSC-1 cellsVirology, 1975
- In vitro synthesis of DNA in nuclei isolated from herpes simplex virus-infected cells, untreated and treated with metabolic inhibitorsVirology, 1975
- Inhibition of Herpes Simplex Virus Replication by Phosphonoacetic AcidAntimicrobial Agents and Chemotherapy, 1974
- Viral Gene Functions Expressed and Detected by Temperature-sensitive Mutants of Herpes Simplex VirusCold Spring Harbor Symposia on Quantitative Biology, 1974
- Suppression of herpes simplex virus infection by phosphonoacetic acid.1973
- The effect of cytosine arabinoside on the replication of herpes simplex virusVirology, 1967