Comparison of oxidative metabolism in vitro in endothelial cells from different species and vessels

Abstract

Oxygen consumption was compared in confluent cultures of endothelial cells from human umbilical cord veins, rat pulmonary arteries, and bovine aortas. A microrespirometric method utilizing oxygenated hemoglobin as oxygen supply and indicator of respiration was used. Respiratory rate was equal in human and murine cells (2.0 × 10⁻⁶ and 2.2 × 10⁻⁶ μl O₂/cell/hour respectively), compared on a cell‐to‐cell basis, while respiratory rate of the bovine endothelium was significantly lower (0.4 × 10⁻⁶ μl O₂/cell/hour) (P < .001).