Role of DNA gyrase in phiX replicative-form replication in vitro.
- 1 May 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (5), 1965-1968
- https://doi.org/10.1073/pnas.74.5.1965
Abstract
Preparations containing DNA gyrase activity were extensively purified from Escherichia coli. Such fractions, in the presence of ATP and Mg2+, catalyze supertwisting of relaxed circular double-stranded DNA replicative forms [RFIV] of a number of DNA that results in the formation of superhelical replicative forms [RFI]. .vphi.X RFIV is not attacked by the A protein endonuclease coded for the .vphi.X DNA genome. After exposure to preparations of DNA gyrase, .vphi.X174 RFIV is converted to .vphi.X RFI which can then be attacked by the .vphi.X gene A protein and participate in replication of duplex .vphi.X DNA.This publication has 18 references indexed in Scilit:
- Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.Proceedings of the National Academy of Sciences, 1976
- DNA gyrase: an enzyme that introduces superhelical turns into DNA.Proceedings of the National Academy of Sciences, 1976
- Restriction assay for integrative recombination of bacteriophage lambda DNA in vitro: requirement for closed circular DNA substrate.Proceedings of the National Academy of Sciences, 1976
- Purification and demonstration of the enzymatic character of the nicking-closing protein from mouse L cellsBiochemical and Biophysical Research Communications, 1976
- Strand and site specificity of the relaxation event for the relaxation complex of the antibiotic resistance plasmid R6KBiochemistry, 1974
- Interactions between twisted DNAs and enzymes: The effects of superhelical turnsJournal of Molecular Biology, 1974
- Isolation and Function of the Gene A Initiator of Bacteriophage ϕX 174, A Highly Specific DNA EndonucleaseProceedings of the National Academy of Sciences, 1974
- Mechanism of transcription of bacteriophage S13: I. Dependence of messengerRNA synthesis on amount and configuration of DNAJournal of Molecular Biology, 1973
- Cis -Limited Action of the Gene-A Product of Bacteriophage ϕX174 and the Essential Bacterial SiteProceedings of the National Academy of Sciences, 1972
- Template activities of the Φη-174 replicative allomorphic deoxyribonucleic acidsBiochemistry, 1971