Studies of Vaccinia Hemagglutinin Obtained from Various Vaccinia Infected Tissues. Density Gradient Centrifugation and Electron Microscopy.

Abstract

Summary Serologically specific vaccinial hemagglutinin (VHA) was obtained from vaccinia infected HeLa cells, chorioallantoic membranes of embryonated eggs and mouse fibroblast cell cultures. When concentrated and partially purified preparations were examined by density gradient centrifugation, the preparations varied in density according to the tissue of origin. The host-associated differences in peak density were accompanied by a marked degree of poly-dispersion not resolvable by a second density gradient centrifugation or by prolonged centrifugation. Electron micrographs showed that the particle size was in the range of 45–54 mμ. Substructure was detected in some particles which suggested the coiling or folding of a strand.