Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA
- 1 March 1976
- journal article
- Published by Springer Nature in Nature
- Vol. 260 (5548), 221-225
- https://doi.org/10.1038/260221a0
Abstract
DNA copies of rabbit globin mRNA have been inserted into the plasmid mini-ColEl. After addition of poly(dT) tails, the plasmid was used to prime the copying of mRNA by reverse transcriptase. Mini-ColEl carrying globin sequences were denatured and annealed with mini-ColEl tailed with poly(dA). Functional plasmids containing the synthesised globin sequences were isolated by transfection in E. coli.Keywords
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