NONPARALLEL SECRETION OF ENZYMES IN HUMAN DUODENAL JUICE AND PURE PANCREATIC-JUICE COLLECTED BY ENDOSCOPIC RETROGRADE CATHETERIZATION OF PAPILLA

Abstract

Duodenal juice was collected from 50 healthy patients, in the basal state, and after i.v. injection of 1 clinical unit of secretin kg-1 + 3 Crick-Harper-Raper units of cholecystokinin-pancreozymin (CCK-PZ) kg-1. Amylase to chymotrypsin and amylase to lipase ratios were significantly decreased by stimulation. Pure pancreatic juice was collected from 7 healthy subjects by endoscopic retrograde catheterization of the papilla. Secretin (1 clinical unit kg-1 h-1) was infused i.v. during 30 min, and a single i.v. injection of CCK-PZ (3 Crick-Harper-Raper units kg-1) was done at the 15th min of the secretin infusion. Chymotrypsinogen and lipase secretions did not parallel that of amylase during CCK-PZ stimulation. As in the 1st set of experiments, amylase secretion was proportionally less stimulated by CCK-PZ injection than were chymotrypsinogen and lipase secretions. In approximately 20% of subjects the secretion of the enzymes was parallel in both duodenal and pancreatic juice.