Nonthermal Atmospheric Plasma Rapidly Disinfects Multidrug-Resistant Microbes by Inducing Cell Surface Damage
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Open Access
- 1 April 2012
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 56 (4), 2028-2036
- https://doi.org/10.1128/aac.05642-11
Abstract
Plasma, a unique state of matter with properties similar to those of ionized gas, is an effective biological disinfectant. However, the mechanism through which nonthermal or “cold” plasma inactivates microbes on surfaces is poorly understood, due in part to challenges associated with processing and analyzing live cells on surfaces rather than in aqueous solution. Here, we employ membrane adsorption techniques to visualize the cellular effects of plasma on representative clinical isolates of drug-resistant microbes. Through direct fluorescent imaging, we demonstrate that plasma rapidly inactivates planktonic cultures, with >5 log10 kill in 30 s by damaging the cell surface in a time-dependent manner, resulting in a loss of membrane integrity, leakage of intracellular components (nucleic acid, protein, ATP), and ultimately focal dissolution of the cell surface with longer exposure time. This occurred with similar kinetic rates among methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Candida albicans. We observed no correlative evidence that plasma induced widespread genomic damage or oxidative protein modification prior to the onset of membrane damage. Consistent with the notion that plasma is superficial, plasma-mediated sterilization was dramatically reduced when microbial cells were enveloped in aqueous buffer prior to treatment. These results support the use of nonthermal plasmas for disinfecting multidrug-resistant microbes in environmental settings and substantiate ongoing clinical applications for plasma devices.Keywords
This publication has 27 references indexed in Scilit:
- Nonthermal Dielectric-Barrier Discharge Plasma-Induced Inactivation Involves Oxidative DNA Damage and Membrane Lipid Peroxidation in Escherichia coliAntimicrobial Agents and Chemotherapy, 2011
- Biological responses of Bacillus stratosphericus to Floating Electrode-Dielectric Barrier Discharge Plasma TreatmentJournal of Applied Microbiology, 2010
- Treatment ofCandida albicansbiofilms with low-temperature plasma induced by dielectric barrier discharge and atmospheric pressure plasma jetNew Journal of Physics, 2010
- Use of atmospheric non-thermal plasma as a disinfectant for objects contaminated with methicillin-resistant Staphylococcus aureusAmerican Journal of Infection Control, 2009
- Non-thermal plasma technologies: New tools for bio-decontaminationBiotechnology Advances, 2008
- Lethal Activity of Nonthermal Plasma Sterilization Against MicroorganismsInfection Control & Hospital Epidemiology, 2008
- Gliding Arc Discharge in the Potato PathogenErwinia carotovorasubsp.atroseptica: Mechanism of Lethal Action and Effect on Membrane-Associated MoleculesApplied and Environmental Microbiology, 2007
- Deactivation ofEscherichia coliby the plasma needleJournal of Physics D: Applied Physics, 2005
- Cell Wall-Deficient Bacteria as a Cause of Infections: A Review of the Clinical SignificanceJournal of International Medical Research, 2005
- Reliability of calcein acetoxy methyl ester and ethidium homodimer or propidium iodide for viability assessment of microbesJournal of Microbiological Methods, 1993