THE LESION OF ACUTE EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS - ISOLATION AND MEMBRANE PHENOTYPES OF PERIVASCULAR INFILTRATES FROM ENCEPHALITIC RAT-BRAIN WHITE MATTER
- 1 January 1984
- journal article
- research article
- Vol. 51 (2), 199-205
Abstract
Vascular elements were isolated from the brain white matter of rats with acute experimental autoimmune encephalomyelitis following transfer of activated myelin basic protein-specific syngeneic T lymphocytes. Fractions containing highly purified capillary and small postcapillary vessels and fractions enriched for perivascular infiltrates (cuffs) were obtained using equilibrium density gradient centrifugation to remove myelin and 1 .times. g sedimentation in a fetal calf serum gradient to sort the vascular elements. Immunocytochemical analysis revealed that capillary cells were negative for Ia determinants. In small postcapillary vessels with scanty lymphoid cell infiltration, Ia expression was restricted to circumscript areas, often, but not always, around adhering lymphoid cells. Immunocytochemistry combined with cytofluorometry established that the majority of all perivascular lymphocytes expressed the phenotype W3/25, defining either helper T cells or T cells involved in delayed-type hypersensitivity. Less than 3% of the lymphocytes were positive for OX8, which defines suppressor and cytotoxic T subsets. Membrane Ig-expressing B lymphocytes were also < 10% of the infiltrating cells.This publication has 27 references indexed in Scilit:
- Demonstration of a soluble mediator that induces exudates rich in Ia-positive macrophages.The Journal of Experimental Medicine, 1980
- Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages.The Journal of Experimental Medicine, 1980
- Two subsets of rat T lymphocytes defined with monoclonal antibodiesEuropean Journal of Immunology, 1980
- Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme ActivityJournal of Neurochemistry, 1980
- Thymic nurse cells. Lymphoepithelial cell complexes in murine thymuses: morphological and serological characterizationThe Journal of Experimental Medicine, 1980
- ISOLATION AND CHARACTERIZATION OF THE INFLAMMATORY INFILTRATE IN THE CENTRAL NERVOUS SYSTEM OF THE GUINEA-PIG WITH EXPERIMENTAL ALLERGIC ENCEPHALOMYELITISNeuropathology and Applied Neurobiology, 1980
- EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS - INHIBITION OF CLINICAL SIGNS AND PARADOXICAL ENHANCEMENT OF LESIONS IN 2ND ATTACKS1980
- Chronic Permeability of the Central Nervous System to Mononuclear Cells in Experimental Allergic Encephalomyelitis in the Lewis RatThe Journal of Immunology, 1978
- CEREBROSPINAL-FLUID LYMPHOCYTES IN EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS1978
- Isolation of metabolically active capillaries from rat brain1,2Journal of Neurochemistry, 1975