The histochemical demonstration of a specific phosphatase (5-nucleotidase)

Abstract
A method was evolved, based on Gomori''s alkaline phosphatase method, for histochemical demonstration of specific phosphatase (5-nucleotidase) activity by incubation with adenosine-5'' -phosphate at pH 7.5. Examples of 5-nucleotidase staining were given, and the limits of localization by this method discussed. In human tissues the strongest 5-nucleotidase staining was found in the posterior pituitary gland.
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