Sensitive Assay for Proteases in Sterile Milk
Open Access
- 1 November 1979
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 62 (11), 1798-1800
- https://doi.org/10.3168/jds.s0022-0302(79)83499-2
Abstract
A sensitive assay for protease activity based on the reaction of primary amino groups of trichloroacetic acid-soluble peptides and amino acids with fluorescamine was applied to sterile milk. The assay was linear in the range of 2-50 nmol of amino groups/aliquot. The assay is suitable for determining proteolytic activity in sterile milk products and for determining protease activity in general.Keywords
This publication has 9 references indexed in Scilit:
- Nachweisgrenzen proteolytischer AktivitätenMicrochimica Acta, 1977
- Low Temperature Inactivation in Milk of Heat-Resistant Proteases from Psychrotrophic BacteriaJournal of Dairy Science, 1976
- Assay of proteins in the presence of interfering materialsAnalytical Biochemistry, 1976
- Heat Resistant Proteases Produced in Milk by Psychrotorophic Bacteria of Dairy OriginJournal of Dairy Science, 1975
- Reduction of Shelf-life of Dairy Products by a Heat-stable Protease from Pseudomonas Fluorescens P26Journal of Dairy Science, 1973
- A fluorescent assay for proteolytic enzymesAnalytical Biochemistry, 1973
- The Action of Proteolytic Enzymes on N,N-Dimethyl ProteinsJournal of Biological Chemistry, 1969
- Assaying Sterile Concentrated Milk for Native Proteolytic EnzymesJournal of Dairy Science, 1964
- Studies on Milk Proteins. II. Colorimetric Determination of the Partial Hydrolysis of the Proteins in MilkJournal of Dairy Science, 1947