Metabolitenprofile von polycyclischen aromatischen Kohlenwasserstoffen nach Vorbehandlung mit verschiedenen Induktoren mikrosomaler Monoxygenasen der Rattenleber

Abstract

The microsomal oxidation of 12 frequently occurring environmental polycyclic aromatic hydrocarbons after incubation with rat-liver microsomes was studied and their metabolites characterized by gas-liquid chromatography/mass spectrometry. The method enables the detection and characterization of phenols, diols, triols and tetrols as trimethylsilyl ethers beside the original hydrocarbons. The induction properties of some carcinogenic and non-carcinogenic hydrocarbons (benz[a]anthracene, pyrene, chrysene, benzo[a]pyrene, benzo[e]pyrene, benzo[b]fluoranthene, benzo[j]fluoranthene, benzo[k]fluoranthene) were studied. Except pyrene and benzo[e]pyrene, all compounds investigated showed significant but different induction factors. The relevance of the induction for an estimation of the biological effect of environmental polycyclic aromatic hydrocarbons is discussed.