Clonal growth of normal and malignant human breast epithelia
- 1 January 1981
- journal article
- research article
- Published by Wiley in Journal of Surgical Oncology
- Vol. 18 (3), 317-322
- https://doi.org/10.1002/jso.2930180313
Abstract
We have developed an efficient method for the growth of colonies from single breast epithelial cells derived from nonmalignant [6], primary [7], and metastatic [1] surgical specimens. The immediate sources of the cells were primary and secondary mass cultures. An enriched culture medium and fibroblast feeder layers were used. A linear relationship between the number of cells cultured and the number of colonies obtained was found. Efficiency of colony formation was higher for those derived from nonmalignant specimens (mean 24%) than those derived from malignant specimens (mean 11%). Fibroblast feeder layers increased the efficiency of colony formation and also supported extended growth of colonies.Keywords
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