The use of a simple method to avoid cell shrinkage during SEM preparation
- 1 February 1981
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 121 (2), 185-189
- https://doi.org/10.1111/j.1365-2818.1981.tb01211.x
Abstract
In the course of preparing specimens for scanning electron microscopy, both glutaraldehyde and OsO4-fixed cells exhibit a considerable shrinkage with a reduction of the mean cellular diameter of about 45% after critical point drying. However, if cells are successively treated with glutaraldehyde, OsO4, tannic acid and uranyl acetate solutions, cellular shrinkage of only 5% is observed.This publication has 9 references indexed in Scilit:
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