Comparative detoxication. 10. The enzymic conjugation of chloro compounds with glutathione in locusts and other insects

Abstract

"Glutathiokinase" activity in insects has been assayed by using the reaction between reduced glutathione and p-nitrobenzyl chloride, 1-chloro-2,4-dinitrobenzene, 1,2-dichloro-4-nitrobenzene or phenoltetrabromophthaleindisulphonate. In locusts, the highest activity was found in fat body, malpighian tubes and gut, with small amounts in other tissues. "Glutathiokinase" activity has been found in seven other insect species and in the cattle tick. The insect enzymes were distinguished from the analogous enzymes in rat, rabbit and cattle tick by their marked sensitivity to inhibition by phthaleins.