AN ACID-SOLUBLE COMPONENT OF THE NUCLEOLUS; THE CYTOCHEMICAL SPECIFICITY OF THE "LEAD ACETATE REACTION"
Open Access
- 1 April 1956
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 4 (4), 331-340
- https://doi.org/10.1177/4.4.331
Abstract
A trichlor-acetic acid-soluble compound resembling inorganic orthophosphate was found in the nucleoli of plant and animal cells. It is not present in the chromosomes or in the chromatin of the interphasic nucleus. This compound precipitates with lead acetate in acetic acid and with an aqueous solution of cobalt acetate; the precipitates are insoluble in pyridine. The "phosphate" of plant nucleoli is not preserved after fixation in chilled acetone or ethanol, but is readily visualized after fixation in a lead acetate solution.Keywords
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