The Successful Production of a Mammalian Tumor with a Virus-Like Principle

Abstract

Yolk sacs of 5-day chick embryos were implanted with saline suspensions of fresh tumor tissue and incubated for 12 days. The yolk was collected, diluted with saline and centrifuged. The supernatant was passed through an N-size Berkefeld filter and when injected sub-dermally (1/3 cc. per mouse) into dba mice caused tumors at the site of inj., in the liver and in the visceral peritoneum.