Use of bio-lac fusion strains to study regulation of biotin biosynthesis in Escherichia coli
- 1 August 1980
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 143 (2), 789-800
- https://doi.org/10.1128/jb.143.2.789-800.1980
Abstract
The technique developed by Casadaban was employed to construct E. coli K-12 derivatives in which the genes determining lactose utilization are fused to the regulatory region of the biotin operon. Fusions of the lac genes to either arm of this divergently transcribed operon were isolated. When the operon is derepressed, expression of the lac genes is sufficient to permit growth on lactose minimal medium. Repressing conditions prevent growth on lactose. This property of bio-lac fusion strains, and the ease of determining the level of operon expression by assaying .beta.-galactosidase, was used for the isolation and characterization of mutants defective in repression. Preliminary analyses of several newly isolated regulatory mutants are presented. For the several birA mutants examined, there appeared to be no direct correlation between effects on minimum biotin requirement and alterations in repressibility, suggesting a possible dual function for the gene. Parallel attempts to obtain fusions of lac to bioH were unsuccessful, indicating lack of direct biotin control at the bioH locus.This publication has 32 references indexed in Scilit:
- Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.Proceedings of the National Academy of Sciences, 1979
- The regulatory region of the biotin operon in Escherichia coliNature, 1978
- Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and MuJournal of Molecular Biology, 1976
- Molecular Biology of Bacteriophage MuScience, 1975
- Operator and promoter mutations affecting divergent transcription in the bio gene cluster of Escherichia coliJournal of Molecular Biology, 1975
- Chromosomal location of mutations affecting the regulation of biotin synthesis in Escherichia coliCanadian Journal of Microbiology, 1975
- Prophage deletion mapping of bacteriophage Mu-1Virology, 1973
- Biotin: Biogenesis, Transport, and Their RegulationPublished by Wiley ,1973
- Sensitive mutants of bacteriophage λVirology, 1961
- Transduction and segregation in Escherichia coli K12Virology, 1957