Über die Komponenten der Dehydrasesysteme. II

Abstract

II. Glucose dehydrase from liver, which Harrison found to be activated by cytochrome + indophenol oxi-dase, was found also to be activated, in the absence of these, by cozymase + flavine-enzyme. Either methylene blue or O acted as acceptor.[long dash]III. The system alcohol dehydrase (or hexose monophosphate dehydrase) [long dash]flavine enzyme[long dash]cozymase[long dash]methylene blue was inhibited by small concns. of Cu, Ag, or Hg, but to a lesser extent by Fe''" or Zn. Monobromoacetate also inhibited, but NaF was without effect.[long dash]IV. The activity of the alcohol dehydrase system was doubled by irradiation with visible light. Red light, which was absorbed in the system only by methylene blue, was most effective. In the presence of light, flavine-enzyme was not necessary, showing that the light reaction was not an acceleration of the normal dehydrogenation process, but the appearance of a new reaction chain. Cozymase was necessary for the light reaction, showing that the cozymase acts on the dehydrase directly, and not on the flavine-enzyme. The light reaction was inhibited by heavy metals in the same manner as the normal reaction.