New human transforming genes detected by a tumorigenicity assay.
Open Access
- 1 September 1984
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 4 (9), 1695-1705
- https://doi.org/10.1128/mcb.4.9.1695
Abstract
We have developed a sensitive bioassay for transforming genes based on the tumorigenicity of cotransfected NIH3T3 cells in nude mice. The assay differs substantially from the NIH3T3 focus assay. Using it, we have detected the transfer of three transforming genes from the DNA of MCF-7, a human mammary carcinoma cell line. One of these is N-ras, which is amplified in MCF-7 DNA. The other two, which we have called mcf2 and mcf3, do not appear to be related to known oncogenes. We cannot detect their transfer by using the NIH3T3 focus assay. We do not yet know whether either mcf2 or mcf3 is associated with genetic abnormalities in MCF-7 cells.This publication has 54 references indexed in Scilit:
- Passage of phenotypes of chemically transformed cells via transfection of DNA and chromatin.Proceedings of the National Academy of Sciences, 1979
- Transformation of mammalian cells with genes from procaryotes and eucaryotesCell, 1979
- The transfer and stable integration of the HSV thymidine kinase gene into mouse cellsCell, 1978
- Packaging recombinant DNA molecules into bacteriophage particles in vitro.Proceedings of the National Academy of Sciences, 1977
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Charon Phages: Safer Derivatives of Bacteriophage Lambda for DNA CloningScience, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Nucleotide sequence of the rightward operator of phage lambda.Proceedings of the National Academy of Sciences, 1975
- A Human Cell Line From a Pleural Effusion Derived From a Breast Carcinoma 2JNCI Journal of the National Cancer Institute, 1973
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973