Photoactivated Conformational Changes in Rhodopsin: A Time-Resolved Spin Label Study
- 26 November 1993
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 262 (5138), 1416-1419
- https://doi.org/10.1126/science.8248781
Abstract
Rhodopsin has been selectively spin-labeled near the cytoplasmic termini of helices C and G. Photoactivation with a light flash induces an electron paramagnetic resonance spectral change in the millisecond time domain, coincident with the appearance of the active metarhodopsin II intermediate. The spectral change is consistent with a small movement near the cytoplasmic termination of the C helix and reverses upon formation of the MIII state. These results provide an important link between the optical changes associated with the retinal chromophore and protein conformational states.Keywords
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