β-Amylase Induction and the Protective Role of Maltose during Temperature Shock

Abstract

A number of studies have demonstrated beta-amylase induction in response to abiotic stress. In the present work, a temperature response profile in 5 degrees C increments from 45 degrees C to 0 degrees C showed that induction at temperature extremes was specific for two members of the gene family (BMY7 and BMY8). Both members encode proteins that possess apparent transit peptides for chloroplast stromal localization. However, induction was not observed for other key starch degrading enzymes demonstrating a rather specific response to temperature stress for BMY7 and BMY8. Time course experiments for heat shock at 40 degrees C and cold shock at 5 degrees C showed that beta-amylase induction correlated with maltose accumulation. Maltose has the ability, as demonstrated by in vitro assays, to protect proteins, membranes, and the photosynthetic electron transport chain at physiologically relevant concentrations. Therefore, beta-amylase induction and the resultant maltose accumulation may function as a compatible-solute stabilizing factor in the chloroplast stroma in response to acute temperature stress.