Androgen induction of messenger RNA concentrations in mouse kidney is post-transcriptional
- 11 March 1986
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 25 (5), 1170-1175
- https://doi.org/10.1021/bi00353a034
Abstract
The concentrations of several mRNAs in mouse kidney increase in response to testosterone. To determine if the increases are generated at the level of gene transcription, we have assayed transcription rates for several androgen-inducible mRNAs in kidney nuclei in vitro. No significant changes were found in the synthesis of three mRNAs whose concentrations increase 10-20-fold during testosterone treatment. Kinetic analysis of changes in transcript levels after testosterone administration and withdrawal suggests that mRNA stabilization is a major factor in the inductions. Thus, the androgen-mediated induction of these kidney mRNAs is generated predominantly at the posttranscriptional level.This publication has 29 references indexed in Scilit:
- RNA inhibits estrogen receptor binding to DNA.Journal of Biological Chemistry, 1981
- Coordinate regulation of two estrogen-dependent genes in avian liver.Proceedings of the National Academy of Sciences, 1980
- Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.Journal of Biological Chemistry, 1979
- An abundant androgen-regulated mRNA in the mouse kidneyCell, 1979
- A Regulatory Locus for Mouse β-Glucuronidase Induction, Gur , Controls Messenger RNA ActivityScience, 1979
- The initiation sites for RNA transcription in Ad2 DNACell, 1977
- MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes.Proceedings of the National Academy of Sciences, 1977
- Glucocorticoid-stimulated accumulation of mouse mammary tumor virus RNA: increased rate of synthesis of viral RNA.Proceedings of the National Academy of Sciences, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: Mapping of nascent RNA molecular labeled in isolated nucleiCell, 1977