Restriction Endonuclease Cleavage Maps of Animal Mitochondrial DNAs

Abstract

The restriction endonuclease, HindIII, gave 3 fragments in each of the 3 mitochondrial DNA isolated from the established mammalian cell lines LA9 (mouse), HeLa (human cervical carcinoma), and BSC-1 (African green monkey kidney). The restriction endonuclease, EcoRI, gave 3 fragments in mitochondrial DNA from HeLa and to 2 in DNA from LA9 and BSC-1. The sizes and the orders of the fragments in the respective genomes were determined with data obtained from the EM. The origin and the direction of replication were designated in each of the cleavage maps. Polyacrylamide gel electrophoretic analyses demonstrated that additional fragments not detectable in the electron microscope and larger than 50 nucleotide pairs were not present.