Isolation and Identification of Infectious Bovine Rhinotracheitis Virus in Tissue Culture

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Abstract
A virus producing a cytopathogenie effect in bovine embryo tissue culture was isolated from cattle having the disease of infectious bovine rhinotracheitis. Employing several strains of the virus, a typical disease could be reproduced in experimental cattle. Protection tests in cattle and serum neutralization tests with sera from naturally and experimentally infected cattle demonstrated that this virus is the etiological agent of infectious bovine rhinotracheitis.